The outermost cuticle of soybean seeds: chemical composition and function during imbibition.

نویسندگان

  • Suqin Shao
  • Chris J Meyer
  • Fengshan Ma
  • Carol A Peterson
  • Mark A Bernards
چکیده

Seeds of different cultivars of Glycine max (L.) Merr. (soybean) have strikingly different rates of water imbibition. Seeds that readily imbibe water are termed 'soft', while those that remain non-permeable, even after several days in water, are referred to as 'hard', 'stone', or 'impermeable' seeds. What prevents soybean hard seeds from taking up water? Previous work established that the initial imbibition of soft soybean seeds correlates with the presence of small cracks in the outermost cuticle that covers the seed coat, prompting a detailed analysis of soybean seed coat cutin. In this paper, it is shown that the outermost cuticle of the seed coat has an unusual chemical composition, lacking typical mid-chain-hydroxylated fatty acids but being relatively rich in other types of hydroxylated fatty acids. The cuticle of the impermeable cultivar studied contained a disproportionately high amount of hydroxylated fatty acids relative to that of the permeable ones. Moreover, a brief treatment with hot alkali released the omega-hydroxy fatty acid component of the outermost cuticle and created holes in it that caused the seeds to become permeable. This demonstrates that the outermost cuticle of the seed is the critical structure that prevents water uptake by hard seeds.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structure and Chemical Composition of Wild Soybean Seed Coat Related to Its Permeability

Four wild and two cultivated soybeans were examined for the water imbibition and relationships with seed coat structure, histochemical and epicatechin content using light, fluorescence, electron microscope, and HPLC technique. Cultivated cultivars, Pungsan and Chungja showed the highest water permeability, while the wild soybean lines, YWS16 and YWS136 showed moderate, and YWS1420 and YWS1459 d...

متن کامل

Raffinose and stachyose metabolism are not required for efficient soybean seed germination.

Raffinose family oligosaccharides (RFOs), which include raffinose and stachyose, are thought to be an important source of energy during seed germination. In contrast to their potential for promoting germination, RFOs represent anti-nutritional units for monogastric animals when consumed as a component of feed. The exact role for RFOs during soybean seed development and germination has not been ...

متن کامل

Effect of Gamma Irradiation on Some Biochemical Parameters and Physico-chemical Properties of Oil in Soybean [Glycine max (L.) Merr.] .

The soybean (Glycine max l. merr) is one of oil seed plants from leguminous family. In this study, the effect of gamma radiation on chlorophyll, soluble and insoluble carbohydrate and composition oil seeds were investigated in soybean. Soybean variety J.K was irradiated with 0,100,200 and 300 gamma ray. The treatment and control seeds planed in with four replicates. The highest amount of total ...

متن کامل

Oxidative Stress Affects [alpha]- Tocopherol Content in Soybean Embryonic Axes upon Imbibition and following Germination.

The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination. Dry, high-vigor axes contained 1.2 [plus or minus] 0.1, nmol/axis and after an increase during the initial 6 h of imbibition, there was a decline to 1.0 [plus or minus] 0.1 nmol/axis at 24 h of incubation. Incubation in the presence of the redox-cycling agent pa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of experimental botany

دوره 58 5  شماره 

صفحات  -

تاریخ انتشار 2007